CYP3A4 and MDR1 copy number analysis by multiplex-PCR analysis (MPA). Ten DNA fragments with sizes ranging from 150 to 501 bp were amplified by PCR using genomic DNA and specific primers: 4 fragments corresponded to CYP3A4 (7q21), 1 to MDR1 (7q21), and 5 to additional fragments used as controls (C-) and located in different chromosomes. (A) The chromatogram of a DNA sample corresponding to PTCL-21 (red) was normalized and compared with a control DNA sample (blue). Fragments showing gains are marked with an asterisk. (B) MPA data were obtained for 11 T-cell lines, the chromatograms for each cell line were normalized with a control DNA sample, and the mean peak fluorescence area was calculated. The values represent the ratio between the T-cell line peak area divided by the control peak area after subtracting 1 plus or minus SD (ie, no gains result in a ratio of 1 and subtraction of 1 will result in a value of 0). Error bars represent SD of 3 independent experiments.