Single H-Y Ag-specific T cells induce CD8⁺ and CD4⁺ T cell–mediated GVHD. (A) Survival in CD8⁺-mediated GVHD after infusion of 1 × 10⁶ CD8⁺ T cells from MataHari donors. Data are from 1 of 4 similar experiments. (B-E) GVHD analysis on day 5. Data are from 1 of 3 similar experiments. (B) Histopathologic analysis of liver (i-ii) and GI tract (iii-iv) by H&E stain (left) and scores (right). (C) Serum levels of TNF-α, IFN-γ, and IL-17A (ND indicates not detected). (D) Allo-H-Y Ag-specific donor CD45.2⁺CD8⁺ T-cell expansion in spleen. (E) Donor CD45.2⁺CD8⁺IFN-γ⁺ in spleen with representative histogram of IFN-γ expression gated by CD45.2⁺CD8⁺ T cells (left) and the absolute number of CD45.2⁺CD8⁺IFN-γ⁺ T cells in spleen (right). (F-G) Survival CD4⁺ GVHD. Data shown are from 1 of 3 similar experiments.