Polyclonal H-Y Ag-specific T cells induce GVHD. (A-B) Experimental schema: polyclonal T cells from WT B6♀ mice that are specific for H-Y Ags were generated after in vivo and subsequent ex vivo priming. These T cells were then transplanted along with TCD BM from B6♀ donors into lethally irradiated syngeneic B6♀ or allogeneic B6♂ animals. The animals were monitored for (C) survival and GVHD score. Data are from 2 combined experiments. **P < .01 compared with female recipients. (D) GVHD histopathology, scores of liver and GI tract and (E) lymphocyte infiltration in the spleen, intestine, and liver. Data are from 1 of 2 similar experiments. (F) Unprimed B6♀ T cells induce GVHD in allogeneic B6♂ recipients. CD90+ T cells (2 × 106 or 15 × 106) donor T cells were harvested from WT B6 female donors and transplanted without priming along with TCD BM into irradiated (11 Gy) syngeneic B6♀ and allogeneic B6♂ animals. The animals were monitored for clinical GVHD severity. (G) Histopathologic analysis, GVHD damage scores of liver and GI tract 70 days after allo-hematopoietic cell transplantation
