Alloantigen expression and GVHD. (A) Experimental schema: the F→F, F→M, and M→F chimeras were generated such that male Ags are either not expressed or expressed only on the target tissues or only on the hematopoietic-derived cells. These chimeras were then irradiated and transplanted with Marilyn T cells along with TCD WT B6♀ BM cells and were monitored for (B) survival and (C) Serum levels of TNF-α, IFN-γ, and IL-17A on day 5. Data are from 1 of 2 similar experiments.