Changes in endothelial permeability induced by P falciparum sonicates in primary HLMECs. Permeability of human lung microvascular endothelial cells (HLMECs) was increased following incubation with parasite sonicates at 10⁷ parasite equivalents/cm² for 24 hours as determined by (A) the flux of FITC-albumin and (B) transendothelial resistance (n = 4). (C) Morphologic changes in ZO-1 staining induced by parasite sonicates as described above for HDMECs (Figure 1A) were also seen in HLMECs. *P < .05, **P < .01 compared with control values by Student paired t test. (A,B) Results are expressed as mean (± SD).