Analysis of the C9-deficient patient with PNH. (A) Clinical profile of the C9-deficient patient with PNH. ND indicates not determined; and PNH erythrocytes, negative for both CD55 and CD59. (B) Correlation between LDH levels and PNH type III erythrocytes (%). ○, 14 patients with PNH; •, C9-deficient patient with PNH; dashed line, upper limit of normal LDH range. (C) C3d expression on erythrocytes of PNH patients with C9 deficiency (C9⁻ PNH), with eculizumab (Ecu⁺ PNH), without eculizumab (Ecu⁻ PNH), and of a patient with autoimmune hemolytic anemia (AIHA). Numbers indicate the population (%) of cells in each quadrant. (D) Arrow indicates a PIGA mutation, deletion of G (352), in the granulocyte genome exon 2 of C9⁻ PNH. (E) Arrows indicate urine hemosiderin stained with Prussian blue of C9⁻ PNH.