Gata3 is required for optimal survival of E2A^−/− lymphomas. (A) Gata3 mRNA expression in Gata3siRNA-expressing lymphomas determined by real-time QPCR. (B) Western blot analysis of Gata3 expression in GFP⁺ 0531 cells infected with Banshee (control), irrelevant siRNA (NSsiRNA), or Gata3siRNA retrovirus. (C) Time course of relative percent GFP⁺ cells after infection of 0531 cells (△, ▴) or 1.F9 cells (○, •) with Banshee (○, △) or Gata3siRNA (•, ▴) retrovirus (left graph), or 0531 cells with Banshee (⊡) and NSsiRNA (♦) retrovirus (right graph). One representative experiment is shown. (D) Relative GFP⁺ cells 5 days after infection of 0531 cells with Banshee, Gata3siRNA, or NSsiRNA retrovirus. The average and standard error of 3 independent experiments is presented. (E) Percent of GFP⁺ cells staining with anti–caspase-3 antibody 24 hours after infection of 1.F9 or 0531 cells with Banshee (□) or Gata3siRNA (▪) retrovirus. Results are the average with standard error of 3 replicate experiments.