Splenic and thymic functions were severely suppressed in recipients with cGvHD. Spleen, thymus, and liver were collected from the recipients with and without cGvHD and TCD BM alone prior to MCMV infection (day 100 after transplantation) and day 10 after MCMV infection (day 110 after transplantation). Lymphocytes were isolated and absolute numbers of nucleated cells per organ were measured by counting the live cells under fluorescence microscopy. Panels A, B, and C represent the absolute number of nucleated cells per spleen, thymus, and liver, respectively (▧, recipients with cGvHD; ▪, recipients with no GvHD; and □, recipients of TCD BM). Panels D, E, and F represent the absolute number of donor spleen T cells per spleen, thymus, and liver, respectively (▧, recipients with cGvHD; ▪, recipients with no GvHD). Panels G, H, and I represent the absolute number of donor BM–derived T cells per spleen, thymus, and liver, respectively (▧, mice with cGvHD; ▪, recipients with no GvHD) that were calculated from the data obtained from the FACS analysis. Data represent an average of 5 to 6 mice were used per group (*P < .05 [Student t test] is for the significant decrease compared with the cell numbers in the spleen and thymus of recipients with no GvHD and TCD BM alone).