Detection of CXCL4 and CXCL4L1 mRNA expression and of CXCL4/CXCL4L1 protein secretion in different human cell types. (A) CXCL4 and CXCL4L1 mRNA levels in platelets (left) and in different human cell types (right). GAPDH was used as a housekeeping gene to correct for small differences in cDNA contents. Total mRNA/cDNA (25 ng) was processed in all experiments. Mean values (± SEM) of triplicate determinations of samples obtained from at least 5 independent donors are reported. (B) CXCL4/CXCL4L1 protein levels in lysate and supernatant from platelets (left) and from different human cell types (right), as assessed by ELISA. Cells were cultured for 24 hours in serum-free medium at a concentration of 1 × 106 cells/mL medium as described in “Materials and methods.” Mean values (± SEM) of duplicates determinations of samples obtained from at least 5 independent donors are reported.
