cell-cycle–dependent expressions of the GFP-GATA2 domain fusion proteins. (A) Scheme of GATA2 structure. *S/T0P+1 motif. (B) Density plot analysis of DNA content and GFP intensity of 293T cells, expressing GFP-GATA2 domain fusion proteins, at 24 hours after transfection with (right) or without (left) nocodazole treatment for the final 14 hours. Percentage of cells in each rectangle region is shown. Histograms demonstrate DNA content of GFP++ cells (in cells with channel > 103, black) and total cells in the same dish (gray). (C) Fluorescent intensity (Y geometric mean channel) of GFP+ (channel > 101) cells. (D-E) DNA content (X geometric mean channel) of GFP++ (channel > 103) cells with (E) or without (D) nocodazole treatment. (C-E)*Significant difference (P < .05) compared with GFP results. Data are shown as means ± standard error of the mean.
