Figure 1
Figure 1. Endothelial cell activation is required for mbKitL expression. (A) Fibroblasts and CDC-HMEC-1 cells were analyzed for the expression of the mbKitL by FACS analysis. Dark lines indicate preimmune mouse IgG used as negative control; light lights, mbKitL expression. (B) RQ-PCR was performed on CDC-HMEC-1 cells treated with LPS, IL-1β, and TNFα for the indicated times. Fibroblasts were used as positive control (+). White lines indicate where irrelevant lanes of the gels have been deleted. (C) CDC-HMEC-1 cells treated with LPS (6 hours), IL-1β, and TNFα (18 hours) were analyzed for the expression of the mbKitL, E-selectin, and ICAM 1 by FACS analysis. Light lines indicate preimmune mouse IgG used as negative control; dark lines, mbKitL, E-selectin, or ICAM 1 expression. Three different experiments were performed with similar results.

Endothelial cell activation is required for mbKitL expression. (A) Fibroblasts and CDC-HMEC-1 cells were analyzed for the expression of the mbKitL by FACS analysis. Dark lines indicate preimmune mouse IgG used as negative control; light lights, mbKitL expression. (B) RQ-PCR was performed on CDC-HMEC-1 cells treated with LPS, IL-1β, and TNFα for the indicated times. Fibroblasts were used as positive control (+). White lines indicate where irrelevant lanes of the gels have been deleted. (C) CDC-HMEC-1 cells treated with LPS (6 hours), IL-1β, and TNFα (18 hours) were analyzed for the expression of the mbKitL, E-selectin, and ICAM 1 by FACS analysis. Light lines indicate preimmune mouse IgG used as negative control; dark lines, mbKitL, E-selectin, or ICAM 1 expression. Three different experiments were performed with similar results.

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