Figure 2
ANCA patterns found with IVIg and IVIg-F(ab′)2 fragments using equimolar concentrations. Flat atypical cANCA pattern identified with IVIg (21 mg/mL; batch A4; titer 1:128; panel A) and IVIg-F(ab′)2 fragments (14 mg/mL; prepared from batch A4; titer 1:128; panel B) as primary antibody, respectively, and FITC-conjugated goat anti–human anti-F(ab′)2 as secondary antibody (top panel) and corresponding negative controls (pooled serum of healthy donors, n = 5; diluted 1:8, bottom panel). A Leica DM RXE conventional fluorescence microscope with a 40×/0.75 PL APO ∞ −0, 17 lens was used.
