Ox/DC-HIL-Fc–treated LN cells display hyperactivation phenotypes. In an independent experiment, draining LN (DLN) cells prepared from BALB/c mice treated similarly (as in Figure 5) were examined. (A) DLN cells were counted. (B) Spontaneous activation was measured by 3H-thymidine incorporation of DLN cells (4 × 105/well) cultured for 3 days without stimuli. (C-D) frequency of leukocytes: DLN cells were stained with FITC-Ab against CD4, CD8, or B220 alone (C) or doubly stained with PE–anti-CD69 (D), and then analyzed by FACS. CD69 expression (D) is shown in LN cells stained positively with the surface marker Ab. Results (A and B) are shown as mean values ± SDs; *P < .001 compared with LN responses treated with hIgG control. Data shown are representative of 3 independent experiments.
