Figure 5
Stat1/Stat3-deficient CD8+ T cells exhibit diminished expansion in response to IL-15 + IL-21. (A) Splenic CD8+ T cells were isolated from control floxed Stat3 mice or Stat1−/−Stat3−/− mice and then Western blotted as in Figure 1A with antibodies to Stat1, Stat3, and Stat5a. (B) Splenic CD8+ T cells were isolated from WT, Stat1−/−, Stat3−/−, or Stat1−/−Stat3−/− mice, stained with CFSE, cultured in complete medium at 5 × 105/mL without cytokine or with 100 ng/mL of IL-15 and/or IL-21 for 4 days, and analyzed by flow cytometry. Percent CFSE dilution is indicated. Flow cytometric results shown are representative of 3 experiments. Statistical analyses are comparisons to cells from WT mice. *P < .05; **P < .01.
