Lymphocytes of various immunotypes in lymph nodes of patients chronically infected with HIV-1 and patients without HIV-1. Phenotype of cells was characterized by flow cytometry after staining with specific antibodies against surface markers at day 1 after surgery. The numbers shown are the percentage of viable T cells. **P < .05 as evaluated by nonparametric Mann-Whitney test. (A) Distribution of lymph node lymphocytes. Boxes represent the 25th-75th percentile of data; medians are indicated, and bars represent the data range of patients with chronic HIV-1 (n = 10; ▪) and patients without HIV-1 (n = 10; □). (B) Ratio of CD4/CD8 lymphocytes. Boxes represent the 25th-75th percentile of data; medians are indicated, and bars indicate the data ranges of the ratio for lymph nodes from 10 chronically infected (left panel) and 10 uninfected (right panel) individuals. For each donor tissue, the number of CD4 and CD8 T cells was measured in 3 pooled tissue blocks and uninfected control.