Thymic gene expression patterns distinguish MG thymuses from healthy subjects. (A) Classical, threshold-based histograms distinguished between extremely changed to unchanged genes in each comparison to newborns (NB). Overrepresented and underrepresented genes (upward, downward arrows) demonstrate the differences between NB thymus to the adult, ML, and MH thymuses. Shown is the percent of changed transcripts in each comparison (y-axis). Compared to NB, adult group increases were less pronounced than ML and MH. Visible extreme expression decreases appeared in the MH group compared with ML and adult groups expressed transcripts (dark green line). (B) Venn diagram presents the number of identified categories using GOdist (significance cutoff of 0.05). Overall, 1600 UniGene clusters had GO mapping with 1264 classified as Biological Process (BP) and 126 as Molecular Function (MF). The nature of change: discrete (light blue, Fisher exact test) or continuous (gray, KS test) and overlaps between detection methods is shown for each group compared with NB. (C) In the MH group, the term “DNA replication and chromosome cycle” decreased specifically compared with its GO parent according to the continuous approach. The change is demonstrated by the CDF plot. The term (green curve) is shifted to the left compared with the parent “cell cycle” (black curve). The change is further supported by the significance of KS test for the low tail (low KS P < .01).