Cytotoxic effect of GX15-070 apoptosis in MCL cells and nomal lymphocytes. (A) Dose response of GX15-070 in MCL cell lines at 20 hours (left panel) and 48 hours (right panel) analyzed by Annexin V–APC staining. (B) Cytofluorimetric analysis of Annexin V–positive cells after treatment of primary MCL cells with the indicated doses of GX15-070 for 20 hours. (C) PBMCs from healthy donors and CD19⁺ cells from reactive tonsils were incubated with the indicated doses of GX15-070 for 20 hours, and viability was analyzed with CD3-FITC/CD19-PE/Annexin V–APC. Results represent the mean ± SD of 3 independent experiments.