Protein expression of Bcl-2 familly members in MCL cells. (A) Total protein extracts (50 μg) from 5 MCL cell lines were analyzed by Western blotting. Membranes were probed for Mcl-1, Bcl-XL, and Bcl-2 expression using suitable antibodies and α-tubulin was used to normalize protein loading. Western blot images are representative results from 3 independent experiments. (B) Relative protein quantification of Mcl-1, Bcl-XL, and Bcl-2 was performed using Image Gauge Fujifilm software.