Figure 2
![Figure 2. Cre-mediated recombination of the Hfeflox allele is highly efficient in both duodenal crypt and tip enterocytes. Indicated cell populations were excised by laser capture microdissection of duodenal sections from HfeVillinCre mice (H&E staining; 10×/0.25 NA objective lens on an Olympus BX51 microscope [Olympus, Hamburg, Germany]). (A) Tip enterocytes (tip), (B) crypt enterocytes (crypt), (C) pure tip enterocytes (p.t.) (D), and pure crypt enterocytes (p.c.). (E) PCR analysis of genomic DNA extracted from samples A to D using primers specific for the floxed (F and R1) or the recombined (F and R2) Hfe allele. The 1-kb (kilobase) Plus DNA ladder (Invitrogen, Karlsruhe, Germany) was used as a size marker.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/10/10.1182_blood-2006-07-036186/4/zh80100701530002.jpeg?Expires=1735501602&Signature=KhiJpKSN0VQlZyHSjl2Nt1W9QoJ7JTAeCGEX~GKnvLZMNXmhV8YR2DPXQgy91ooc623qyUo0aLW7cHCKUDHNBKrJJsDnFCs5Kl0MDl~tdobXl2ojqSwycUSTIyeg~EjhH5h2u7kQ41~z5dFzV~NBq1-0snc-yHX1nbhxVOCfG554o5qugm110bc0nUnYikWzt2n3r3qQhLp9PhkOJw0vSXOt3qK3A6ERrehx67-URYTl6K2GovU61VQhXHxN6sCSUEsa0RRH0lIl0okH6rUqm-zv2L6N0lF~qA7JUc~8WnjObvmNdDpkP6j0vEsZDwr1nAUWv~KWYkLhSZZSVyHfCw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Cre-mediated recombination of the Hfeflox allele is highly efficient in both duodenal crypt and tip enterocytes. Indicated cell populations were excised by laser capture microdissection of duodenal sections from HfeVillinCre mice (H&E staining; 10×/0.25 NA objective lens on an Olympus BX51 microscope [Olympus, Hamburg, Germany]). (A) Tip enterocytes (tip), (B) crypt enterocytes (crypt), (C) pure tip enterocytes (p.t.) (D), and pure crypt enterocytes (p.c.). (E) PCR analysis of genomic DNA extracted from samples A to D using primers specific for the floxed (F and R1) or the recombined (F and R2) Hfe allele. The 1-kb (kilobase) Plus DNA ladder (Invitrogen, Karlsruhe, Germany) was used as a size marker.
