Figure 4
![Figure 4. Gfi1b expression in early precursor and hematopoietic stem cells. (A-C) Bone marrow cells from wt or wt/KI (Gfi1b+/GFP) mice, respectively, were depleted for lineage-positive cells (Lin: CD3, CD4, CD8, Mac-1, B220, Ter119, Ly6G, Pan natural killer [NK] plus Sca1, IL7Rα for CMP-GMP-MEP analysis), and negative cells were restained for surface markers as indicated and analyzed by flow cytometry (A-C, left panels). HSCs (A), MEPs, CMPs, GMPs (B), or CLPs (C) were electronically gated as previously described (see “Material and methods”) and analyzed for GFP expression (right panels).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/6/10.1182_blood-2006-06-030031/4/zh80060709530004.jpeg?Expires=1736050591&Signature=pc1-~LC43PW3n6illP8cyZHaNZ44KPTPqK~f7jJju82Af~iPDMXLwrdCappGttdN2sxOvBQor9EJGKrGPNUpnM5yCnofVl38WWlaSBJrBDMVRzlulnVfmJGIw~v8moUuNJsNdDFiKRzuoXaSggnX0~G99RYDj1c~Ian0yPLpCzk6f52G9vTVCEIpqfFFs9D6grNsLN9R~WCYdZEEtWgTJvBVLjJlvlI5Dr~xEx7KViw5olVT15l9VhNINjYPdLeUpazlDHqtCyR5CGsPx-BYJ0CyrlSGDPIT-lXmwUiQ-9pQOK-61CUdVRy3yUqfW773NN8fPQzS2ryzIikfIq7iTw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Gfi1b expression in early precursor and hematopoietic stem cells. (A-C) Bone marrow cells from wt or wt/KI (Gfi1b+/GFP) mice, respectively, were depleted for lineage-positive cells (Lin: CD3, CD4, CD8, Mac-1, B220, Ter119, Ly6G, Pan natural killer [NK] plus Sca1, IL7Rα for CMP-GMP-MEP analysis), and negative cells were restained for surface markers as indicated and analyzed by flow cytometry (A-C, left panels). HSCs (A), MEPs, CMPs, GMPs (B), or CLPs (C) were electronically gated as previously described (see “Material and methods”) and analyzed for GFP expression (right panels).
