AML1-ETO promotes leukemia in p21Waf1-deficient mice. (A) Kaplan-Meier survival plot of mice receiving transplants of MigR1- or Mig-A/E–infected p21Waf1 cells. Mice were monitored for leukemia development 3 weeks after transplantation. (B) Hematoxylin and eosin staining of liver and spleen of a control mouse and a leukemic mouse, showing the disruption of the tissue architecture by infiltrating leukemic cells. (C) Accumulation of blast cells derived from AML1-ETO–expressing p21Waf1-deficient cells in hematopoietic compartments. Cells isolated from spleen and bone marrow from a recipient mouse of transplanted p21WAF1-deficient cells expressing AML1-ETO and a control were cytospun and stained with Wright-Giemsa for the analysis of immature blasts indicated by the arrows. Microscopy was performed with a Leica DMLB microscope (Leica Microsystems, Wetzlar, Germany) using an HC PLAN 10×/22 eyepiece and 10×/0.25 or 20×/0.4 objective lenses, or a 100×/1.30 oil-immersion objective lens (Richard Allan Scientific, Kalamazoo, MI). Pictures were captured using Spot software from Diagnostic Instruments (Sterling Heights, MI).
