Temporal emergence of VE-cadherin⁺α4-integrin⁺ cells during ESC differentiation. As described for Figure 1, undifferentiated GFP-transfected ESCs and subsequent differentiating cells were analyzed by FACS. Percentages of cells in each quadrant among the total viable GFP⁺ cells are indicated. Representative results from 1 of 3 independent experiments are shown.