Effect of caspase inhibition on MRP8/MRP14-induced apoptotic and necrotic alterations. HMEC monolayers were left either untreated (ct) or were stimulated with 200 μg/mL MRP8/MRP14 (MRP), 1 μg/mL ActD, or 200 nM STS in the absence (□) or presence of 40 μM (⊡) or 100 μM zVAD-fmk (▪). After 24 and 48 hours, cells were analyzed for the percentages of hypodiploid cell nuclei (A) and PI-positive cells (B) by flow cytometry. The results represent means ± SEM from 6 independent experiments. *Significant inhibition by zVAD-fmk (P < .05).