Effects of MRP8/MRP14 on the endothelial integrity. (A) HMEC monolayers were left untreated (⊡) or treated with 200 μg/mL MRP8/MRP14 (▪). After the indicated time periods, TER was determined as described in “Materials and methods.” (B) HMEC monolayers were left untreated or treated for 16 hours (⊡) and 24 hours (▪) with 200 μg/mL MRP8/MRP14 in the presence of the caspase inhibitor zVAD-fmk (40 μM and 100 μM) and analyzed for TER. *Significant decrease of the TER by following MRP8/MRP14 treatment (P < .05). Error bars indicate standard deviations in 3 independent experiments.