The death receptor pathway is not involved in MRP8/MRP14-induced cell death. The time course of apoptosis (A) and necrosis (B) was analyzed in Jurkat cells that were stimulated with MRP8/MRP14 or left untreated similarly to HMECs (Figure 2). *Significant differences between treated and untreated cells (n = 5; P < .05). (C-D) Wild-type (wt), caspase-8–deficient, and FADD-deficient Jurkat cells were left untreated (□) or were incubated for 24 hours (C) and 48 hours (D) with MRP8/MRP14 (▪) or 20 ng/mL TNFα and 1 mM cycloheximide (⊡) and analyzed for the percentage of apoptotic cells with hypodiploid nuclei. The data represent the means ± SEM from 4 independent experiments. *Significantly increased amounts of apoptotic cells compared with control cells (P < .05).