American Society of Hematology

Figure 4

$Figure 4. CRTH2+ Th cells exhibit an “enhanced” Th2 phenotype. (A) Two-color staining for IL-4 and CCR5 or CRTH2 in Th2-skewed cells restimulated and monensin-treated as in Figure 2B. (B) Seven-day Th2 cultures were sorted by CRTH2 expression for further phenotypic analysis. The resulting preparations are referred to as “CRTH2+” and “CRTH2−.” (C) IL-4 and IFN-γ levels were measured by ELISA of supernatants collected from either preparation after 3-day subculture and 20-hour stimulation with PMA and ionomycin and expressed as percent of the levels detected in samples of the parental Th2 cultures left unfractionated and stimulated in parallel. Shown are the means ± SEM of 5 independent experiments. Differences in the expression of IL-4 and IFN-γ in CRTH2− versus CRTH2+ cultures were statistically significant (P < .05).$

CRTH2⁺ Th cells exhibit an “enhanced” Th2 phenotype. (A) Two-color staining for IL-4 and CCR5 or CRTH2 in Th2-skewed cells restimulated and monensin-treated as in Figure 2B. (B) Seven-day Th2 cultures were sorted by CRTH2 expression for further phenotypic analysis. The resulting preparations are referred to as “CRTH2⁺” and “CRTH2⁻.” (C) IL-4 and IFN-γ levels were measured by ELISA of supernatants collected from either preparation after 3-day subculture and 20-hour stimulation with PMA and ionomycin and expressed as percent of the levels detected in samples of the parental Th2 cultures left unfractionated and stimulated in parallel. Shown are the means ± SEM of 5 independent experiments. Differences in the expression of IL-4 and IFN-γ in CRTH2⁻ versus CRTH2⁺ cultures were statistically significant (P < .05).

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