Intrinsic apoptosis-suppression uncouples somatic cells, while differentiation of mESCs in the presence of LIF does not prevent coupling. Ba/F3 cells and their derivatives containing an expression vector for overexpressing anamorsin were treated with control solvent or nocodazole or etoposide for 48 hours, then harvested and cell cycle/apoptosis analysis performed (A) as in Figure 2A. Percentages of polyploidy and apoptosis (mean ± 1SD) from 3 experiments is shown. (B) MO7e cells expressing empty vector or a vector containing survivin. Cells were treated with paclitaxel or control solvent for 48 hours then harvested, and cell-cycle analysis was performed. This experiment was repeated once with similar results. (C) E14 mESCs were treated with RA for 3 days, than treated for 1 additional day with nocodazole added. SSEA-1 expression is shown in the top panel, and cell-cycle analysis of SSEA-1-Hi and SSEA-1-Lo gated cells is shown in the bottom panel. (D) Caspase-3 activation in cultures from panel C.