Colonies of the hESC line HSF-6 also contain 2 populations that differ in marker expression and nocodazole-induced polyploidy. Colony edge of HSF-6 (A) and hEB (B) formation (Nikon Diaphot; panel A, 10 × 40; panel B, 10 × 20). HSF-6 colonies contain 2 populations based on laser-light scatter pattern (C) analogous to MI01 (Figure 5D). (D-E) Pluripotent marker expression along with nonspecific isotype control antibody binding. HSF-6 colonies were harvested and analyzed as in Figure 5. Cell-cycle analysis of gated hESC-A and hESC-B populations after treatment with nocodazole (F) was done as in Figure 5. Data represent 2 independent experiments.