Effect of PKC inhibition on monocyte progenitor frequency and colony formation. Day-1 cells were transduced in the presence of the indicated concentration of GF109203X. (A) GFP⁺ cells were assessed for M-CSFR expression on day 3 as shown in Figure 2A. Error bars represent SD (n = 3); *P ≤ .05. (B) Frequency distribution of colony formation of day 3 cells was assessed as described in Figure 1 (n = 3). G indicates granulocytic; M, macrophage; GM, granulocyte/macrophage.