Activated PDK1 mimics the effect of Ras on colony formation and PKC activation. (A) Effect of PDK1 and myrPDK1 on colony formation of CD34⁺ cells. Conditions as in Figure 1 (n = 4). (B) Western blot analysis of cytosolic and membrane components from the indicated cultures (day 7). Figures indicate mean relative expression (from 4 replicate experiments) compared to equivalent control cells. *P ≤ .05, **P ≤ .01 (see also note in Figure 4). (C) Effect of Ras and myrPDK1 on the phosphorylation of Akt and Erk. Representative Western blot data of total protein from day 7 cultures probed with the indicated antibody. Data are representative of 3 independent experiments.