PDK1 and phospho-PKC are overexpressed in patients with AML. (A) Western blot data from normal bone marrow subpopulations and AML blasts fractionated into cytosolic and membrane components. Figures show expression levels relative to normal CD34⁺ bone marrow cells for which representative data are shown (n = 4); see also note Figure 4. (B) Quantitative analysis of total PDK1 and phospho-PKC expression in AML blasts (normalized to mean expression in CD34⁺ cells; n = 5). Dashed lines indicate statistical threshold of normal expression (mean ± 2 SD of normal CD34⁺ cells). (C) Expression of PDK1 related to AML FAB subtype.