Cytokine production by XLA-DCs and DCs from healthy controls after stimulation with TLR agonists. (A) Relative production of TNFα, IL-6, IL-12 p70, and IL-10 by XLA-DCs, control DCs, and control DCs preincubated with 100 μM LFM-A13 after stimulation with TLR agonists. Data are expressed as relative production as compared with control DCs. Bars show means/SD of 4 different patients and healthy controls. *P value for comparison with control DCs; P < .05. (B) IL-6 and TNF- α production by XLA-DCs and DCs generated from healthy controls after ssRNA stimulation. Dots represent individual patients; horizontal bars, means. The Mann-Whitney test was used for statistical analysis. (C) The effect of Btk inhibitor LFM-A13 (at 2 different concentrations) on the production of TNF-α and IL-6 by DCs generated from 5 healthy blood donors. Representative results of 5 independent experiments are shown.