Transcription, translation, and expression of wt and Cys-modified TCR chains. (A) The T-cell clone CE10 (bold line), human mock-transduced T cells (thin line), and T cells transduced with the vα21_wt and vβ21_wt (dotted line) or vα21_Cys and vβ21_Cys TCR chains (dashed line) were stained with an anti-vβ21–FITC antibody. (B) Vα21 and vβ21 chain RNA from T cells transduced with the vα21_wt and vβ21_wt or vα21_Cys and vβ21_Cys TCR chains was quantitated by TaqMan RT-PCR and normalized to β-actin message. Error bars indicate SEM. (C) Mock-transduced T cells (thin line) and clone CE10 (bold line) were treated with paraformaldehyde and stained with the anti-vβ21–FITC antibody. (D) The T-cell clone CE10 (bold line), mock-transduced T cells (thin line), and T cells transduced with vα21_wt and vβ21_wt (dotted line) or vα21_Cys and vβ21_Cys TCR chains (dashed line) were treated with paraformaldehyde, permeabilized, and stained with the anti-vβ21–FITC antibody.