Increased surface expression of Cys-modified TCR chains is not unique to a single TCR αβ pair and requires presence of a corresponding cysteine on both chains. (A) Mock-transduced T cells (thin line) and T cells transduced with vα4_wt and vβ13_wt (dotted line) or vα4_Cys and vβ13_Cys TCR chains (dashed line) were stained with anti-vβ13–FITC antibody. (B) Mock-transduced T cells (thin line) and T cells transduced with vα21_wt and vβ13_wt (dotted line) or vα21_Cys and vβ13_Cys TCR chains (dashed line) were stained with anti-vβ13–FITC antibody. (C) Mock-transduced T cells (thin line) and T cells transduced with vα4_wt and vβ13_wt (thick line) or vα4_wt and vβ13_Cys TCR chains (dashed line) were stained with anti-vβ13–FITC antibody. (D) Mock-transduced T cells (thin line) and T cells transduced with vα21_wt and vβ21_wt (thick line) or vα21_wt and vβ21_Cys TCR chains (dashed line) were stained with anti-vβ21–FITC antibody. (E) Mock-transduced T cells (thin line) and T cells transduced with vα21_wt and vβ21_wt (thick line), vβ21_wt TCR chain alone (dotted line), or vβ21_Cys TCR chain alone (dashed line) were stained with anti-vβ21–FITC antibody. (F) Mock-transduced T cells (thin line) and T cells transduced with vα21TAG_wt and vβ21_wt (thick line), vα21TAG_wt TCR chain alone (dotted line), or vα21TAG_Cys TCR chain alone (dashed line) were stained with anti-TAG–PE antibody. All results are representative of a least 3 independent experiments.