Resveratrol suppresses AKT activation and inhibits the expression of antiapoptotic proteins in MM cells. (A) U266 cells (2 × 106/mL) were treated with 50 μM resveratrol for the indicated times, and whole-cell extracts were prepared, separated on SDS-PAGE, and subjected to Western blotting with a phospho-AKT antibody. The same blot were stripped and reprobed with AKT antibody to show equal protein loading. (B) U266 cells (left)/MM1.S (middle)/RPMI 8826 (right) were treated with 50 μM resveratrol for the indicated times, and whole-cell extracts were prepared, separated on SDS-PAGE, and subjected to Western blot analysis using cyclin D1 antibody. The same blot was stripped and reprobed with β-actin antibody to verify equal protein loading. (C) U266/MM1.S/RPMI 8826 cells were treated as described in panel A, and whole-cell extracts were prepared, separated on SDS-PAGE, and subjected to Western blot using antibodies against indicated proteins. The same blots were stripped and reprobed with β-actin antibody to show equal protein loading. The results shown are representative of 3 independent experiments.
