Impaired proliferation and survival and decreased expression of maturation markers in RhoH-null thymocytes. (A-B) Thymocytes of 2-month-old mice were analyzed for proliferating BrdU-incorporating cells and apoptotic AnnexinV⁺ cells. Different thymocyte populations were distinguished (see Figure 1A-B). *P < .05; **P < .01; ***P < .001; BrdU, DN1-DN4, n [control]/[RhoH^−/−]: 6/10; DP, CD4SP, CD8SP, n [control]/[RhoH^−/−]: 8/10; Annexin V, DN1 to DN4, n [control]/[RhoH^−/−]: 10/10; DP, CD4SP, CD8SP, n [control]/[RhoH^−/−]: 8/9. (C-E) Thymocytes of 2-month-old mice were analyzed for the expression of CD4, CD8, and CD5 (C; n [control]/[RhoH^−/−]: 9/9) or TCRβ (D; n [control]/[RhoH^−/−]: 4/4) or CD69 (E; n [control]/[RhoH^−/−]: 6/6) by FACS. Percentages of cells marked in histograms are shown in graph. **P < .01; ***P <.001. Error bars show standard deviation.