![Figure 4. Defective positive selection in the absence of RhoH. (A) Thymocytes of 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR, either expressing or not expressing RhoH, were analyzed for the expression of CD4 and CD8 by FACS (upper panel). Quantification of absolute numbers of thymocyte subpopulations (lower panel). *P < .05; **P < .01; ***P < .001. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 3/4). (B) Thymocytes of 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR, either expressing or not expressing RhoH, were gated for lineage-negative (B220, CD4, CD8, NK1.1, Mac1, Gr-1, Ter119) cells and analyzed for the expression of CD25 and CD44. DN1, CD25−CD44+; DN2, CD25+CD44+; DN3, CD25+CD44−; DN4, CD25−CD44−; upper panel). Quantification of absolute numbers of thymocyte subpopulations (lower panel; *P < .05. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 3/4). (C) Decreased amount of Vα2+ DN thymocytes in 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR in the absence of RhoH. *P < .05; **P < .01. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 4/4). (D) Increased amount of CD5low thymocytes in 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR in the absence of RhoH. **P < .01; ***P < .001; Error bars indicate the standard deviation (n [control]/RhoH−/−]: 4/4). (E) Decreased amount of CD69high thymocytes in 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR in the absence of RhoH. *P < .05; **P < .01. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 4/4).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/6/10.1182_blood-2006-04-019034/4/zh80060709420004.jpeg?Expires=1736046463&Signature=SxDix1P~XDmUfQd0exH7qNqOoRDHKNH-KlnOG6zrG-GTpDwywrFOLB02tRAmkDXRogQORaCwHngm3bHkE2bUyZMNX~orm9jq3dxt8ck6f9WFEt~P8LxJ2F2lLVTUD0p8x-lMt0kDhCj5NBVfWNR~gNbxpTnOEqWhiZpxZneL50GirNz8C2-hQy~w1pECZQDgW3PQeQXoJ7zJell-riaq2PLSIpiTy1UHJz68CrXze8KmslyFoQnCyrIkENx1OwSCaJAikCv-yxfFmj~gH3hN90GTWJ9VuxdfOk0XX2rKicuuI3JXvTsnn2quKIEpQUe1RuO4OBfH02DMbIY09l0E-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Defective positive selection in the absence of RhoH. (A) Thymocytes of 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR, either expressing or not expressing RhoH, were analyzed for the expression of CD4 and CD8 by FACS (upper panel). Quantification of absolute numbers of thymocyte subpopulations (lower panel). *P < .05; **P < .01; ***P < .001. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 3/4). (B) Thymocytes of 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR, either expressing or not expressing RhoH, were gated for lineage-negative (B220, CD4, CD8, NK1.1, Mac1, Gr-1, Ter119) cells and analyzed for the expression of CD25 and CD44. DN1, CD25−CD44+; DN2, CD25+CD44+; DN3, CD25+CD44−; DN4, CD25−CD44−; upper panel). Quantification of absolute numbers of thymocyte subpopulations (lower panel; *P < .05. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 3/4). (C) Decreased amount of Vα2+ DN thymocytes in 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR in the absence of RhoH. *P < .05; **P < .01. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 4/4). (D) Increased amount of CD5low thymocytes in 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR in the absence of RhoH. **P < .01; ***P < .001; Error bars indicate the standard deviation (n [control]/RhoH−/−]: 4/4). (E) Decreased amount of CD69high thymocytes in 4- to 7-week-old OT-II mice transgenic for an ovalbumin-specific TCR in the absence of RhoH. *P < .05; **P < .01. Error bars indicate the standard deviation (n [control]/RhoH−/−]: 4/4).
