The redox state of Prx2 in 5 μM H₂O₂-treated erythrocytes over time. (A) A representative immunoblot of the redox transitions of Prx2 following a bolus treatment with 5 μM H₂O₂. (B) Extracellular H₂O₂ levels over time after a bolus treatment of 5 μM H₂O₂. Data are the means ± SD from 3 experiments. (C) Jurkat cells were pretreated with 30 μM DNCB for 10 minutes prior to the indicated concentrations of H₂O₂, then immunoblotted (30 μg protein per lane). (D) The inhibition of erythrocyte Prx2 regeneration by treatment with 30 μM DNCB prior to 5 μM H₂O₂. D indicates dimeric Prx2; M, monomeric Prx2.