Survivin protein is increased in trisomy 8 CD34 cells. Flow cytometry was performed on column-purified CD34 cells stained by intracellular staining as described in “Materials and methods” using survivin-FITC mAb. Survivin protein was increased in samples obtained from trisomy 8 BM (example seen in panel A). Entire sampling of patients examined is seen in panel B. Immunoblots were performed on total-cell lysates of BMMNCs for survivin. Increases in patients with trisomy 8 compared with healthy controls (n = 2) or other patients with MDS (n = 6) could be seen. Error bars represent SEM.