Absence of significant DNA degradation in annexin⁺ cells from patients with trisomy 8. (A) Cell-cycle analysis of sorted CD34 annexin⁺ and annexin⁻ cells showed that trisomy 8 cells were more heavily represented in the annexin⁺ fraction, but no significant numbers of cells with subgenomic DNA could be seen in this fraction (full dataset in Table 1). (B) When purified CD34 cells were stained with annexin-PE and CD34-FITC, or the TUNEL assay performed, there was significant annexin staining, but few cells were TUNEL⁺ (full dataset in Table 1).