![Figure 3. Representative histological appearance of spleen and liver in BALB/c mice injected with 100 μg of 34-3C IgM, IgA, and IgG2a anti-RBC mAb. Note an enormous accumulation of agglutinated RBC in red pulp of spleen 2 days after injection of polymeric forms of IgM or IgA anti-RBC mAb (A, IgM; B, IgA; C, IgG2a; hematoxylin & eosin [H&E], original magnification ×40), focal necrotic lesions of hepatic parenchymal cells in liver 8 days after injection of IgM anti-RBC mAb (E,F; H&E, original magnification ×40 and ×200), and extensive iron deposits by Kupffer cells in liver 8 days after injection of IgG2a anti-RBC mAb (G, IgM; H, IgA; I, IgG2a; Perls iron staining, original magnification ×200). As a control, normal histological appearances of spleen and liver from mice injected with 500 μg of monomeric form of 34-3C IgM anti-RBC mAb are shown (D,J).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/109/12/10.1182_blood-2006-11-059899/4/zh80120701330003.jpeg?Expires=1735823697&Signature=dXgSCRqb2YbNS1xxTVLfEPJVZeKAJRKM1ulghIorx2eYedJTMpjcKEiCVp7OD8Dxz6z6RaySpSgVeOFT8LAgYkUz-52Qyzj2cN0ya~Ig-v0N93VNYR9BRtpnt3qChTBj3ccPo9laqOeJUia1SZBsiiY~2WrbCkJP~seM5H2mLcukZyXMrp0xWZbvxnuN-bTZvq-LSLPchL8lX79KzW9e71Bms8AUEHBkQLPkJeEtzP2AiUI2~SVSznJzew0a2Fks6eNHV0F~hZJ173FJzk6I213xMTk6waOYYp2r3uHC97-Tp4TTRTp0vfbipL9Gvu2Yh-rP4J2OlLd~A3FmFJUqSQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Representative histological appearance of spleen and liver in BALB/c mice injected with 100 μg of 34-3C IgM, IgA, and IgG2a anti-RBC mAb. Note an enormous accumulation of agglutinated RBC in red pulp of spleen 2 days after injection of polymeric forms of IgM or IgA anti-RBC mAb (A, IgM; B, IgA; C, IgG2a; hematoxylin & eosin [H&E], original magnification ×40), focal necrotic lesions of hepatic parenchymal cells in liver 8 days after injection of IgM anti-RBC mAb (E,F; H&E, original magnification ×40 and ×200), and extensive iron deposits by Kupffer cells in liver 8 days after injection of IgG2a anti-RBC mAb (G, IgM; H, IgA; I, IgG2a; Perls iron staining, original magnification ×200). As a control, normal histological appearances of spleen and liver from mice injected with 500 μg of monomeric form of 34-3C IgM anti-RBC mAb are shown (D,J).
