Elution profiles of polymeric and monomeric forms of 34-3C IgM or IgA mAb on Sephadex G200 gel filtration column. Polymeric (•) and monomeric (○) forms of 34-3C IgM (A) and IgA (B) mAb were individually subjected to a Sephadex G200 column equilibrated with 50 mM phosphate buffer (pH 7.4) containing 0.15 M NaCl, and elution was performed using the same buffer at a flow rate of 0.2 mL/min at 4°C. The column was calibrated with blue dextran (void volume), IgG (150 kD), and BSA (67 kD), and their positions are indicated by arrows: 1, void volume; 2, IgG; 3, BSA.