Gene expression in MCL-BV. (A) Demonstration of IL-14α expression in MCL-BV cell lines and fresh tumors from various patients, performed as described in “Patients, materials, and methods.” (B) Semiquantitative RT-PCR analysis of bcl-2 and Pak expression in spleen from C57BL/6 mice, and tumors from IL-14a TG, DTG, and c-Myc TG mice, performed as described in “Patients, materials, and methods.” NTC indicates negative control (no primers). (C) Expression of NF-κB components in the lymphoma of a DTG mouse. Total RNA purified from the indicated mouse was analyzed for NF-κB1, NF-κB2, p65, and RelB by semiquantitative RT-PCR. (D) Translocation of NF-κB to the nucleus in DTG lymphoma cells. EMSA analysis was performed as described in “Patients, materials, and methods” using nuclear extracts purified from Mino cells or DTG lymphoma cells. FP indicates free probe. (E) Cytoplasmic and nuclear extracts were purified from Mino cells or 3 different cell lines derived from the lymphoma of DTG mouse and analyzed for NF-κB components by Western blotting as described in “Patients, materials, and methods.”