PLMCs are HIV infection resistant. (A) FMF analysis of IgE-selected and purified (CD117⁺ in green). PLMCs revealed a lack of cell surface–expressed CD4, CCR5, and CXCR4 (HIV) viral receptors and coreceptors relative to controls (in red). Black lines demarcate cell populations treated with antigen-specific antibody; white lines demarcate cell populations treated with isotype-matched control. (B) No detectable evidence by PCR of viral entry (SS cDNA) in PLMCs after challenge with either R5-tropic (HIV_BAL) or with X4-tropic (HIV_TYBE); HIV_BAL-infected LAD-2 + cells or non (virus) treated (NT) were included as controls. All experiments were performed a minimum of 3 times. All error bars represent standard deviation of the mean of replicate PCR reactions at each data point.