Conditioned media derived from VLP-treated PBMCs or MDMs inhibit HIV replication in CD4⁺ T cells. Control-Sup (□) and VLP-Sup (▪) from PBMCs (A) and MDMs (B) were collected as described in “Materials and methods.” PHA-stimulated CD4⁺ T cells were infected with X4 virus and then cultured in the presence of different percentages of the Sup for 7 days. Half of culture supernatants were changed on the 4th day with fresh culture media alone. HIV-1 replication was measured by p24 antigen capture assay. Data show means ± SDs. Representative results from 3 independent results are shown.