Activation with anti–CD3 + IL-2 and up-regulation of perforin did not enhance the anticryptococcal activity of CD4⁺ T cells from healthy adults.(A) CD4⁺ T cells were activated with anti–CD3 + IL-2; Western blot was performed for granulysin and perforin. (B) CD4⁺ T cells were activated with anti–CD3 + IL-2 or IL-2, and the number of C neoformans was determined as indicated. Results are expressed as mean ± SEM. *P < .01 compared with the number of C neoformans alone. Data are representative of 3 independent experiments.