Granulysin-mediated anticryptococcal activity did not require perforin to function. (A) CD4⁺ T cells were activated with anti–CD3 + IL-2 and treated with CMA to deplete perforin. Expression of perforin and granulysin was assessed by Western blot. (B) CD4⁺ T cells were activated with anti–CD3 + IL-2 and treated with CMA. The number of C neoformans was determined as indicated. Results are expressed as mean ± SEM. *P < .01 compared with the number of C neoformans alone at 24 or 48 hours. Data are representative of 3 independent experiments.