Granulysin is responsible for the anticryptococcal activity of anti–CD3 + IL-2–activated CD4⁺ T cells from healthy donors. (A) Granulysin or perforin steady-state mRNA was assessed by RT-PCR from CD4⁺ T cells silenced with granulysin siRNA (G1) or control siRNA as described in Figure 5A. (B) Corresponding granulysin and perforin were detected by Western blot. (C) The anticryptococcal activity of activated CD4⁺ T cells from healthy donors was determined as indicated. *P < .01 compared with the number of C neoformans alone at 24 or 48 hours. Data are representative of 3 independent experiments. (D) Granulysin or perforin steady-state mRNA was assessed by RT-PCR from CD4⁺ T cells treated with a different siRNA to granulysin (G2) or the control siRNA. Data are representative of 2 independent experiments. (E) The corresponding anticryptococcal activity of activated CD4⁺ T cells from healthy donors was determined. Results in panels C and E are expressed as mean ± SEM. *P < .01 compared with the number of C neoformans alone at 24 or 48 hours. Data are representative of 2 independent experiments.