Response to rapamycin in cell culture. (A) BC-1 or (B) BCBL-1 cells were seeded in medium containing the indicated concentrations of rapamycin. Plotted is the cell number on the vertical axis and time after seeding on the horizontal axis. This data represents 1 of 2 biologic replicates. (C-F) Cell-cycle analysis using propidium iodide stain at 4 days after exposure to 50 nM rapamycin or mock. In each panel plotted on the horizontal axis is the relative DNA content and on the vertical axis the number of cells. Also shown is the percentage of cells with ≤ G₁, G₁, S, G₂ and ≥ G₂ DNA content for each of the indicated cell lines and conditions.