Rapamycin does not affect KSHV transcription but cellular cytokine levels. Shown for each panel A to F on the vertical axis are fold changes relative to mRNA levels at t = 0 on a log10 scale and on the vertical axis days after addition to 50 nM rapamycin or vehicle control (mock). Rapamycin-treated cultures are represented by black squares, mock-treated cultures by gray circles. The results of rapamycin-resistant BCBL-1 cells are shown on the left column and of rapamycin-sensitive BC-1 cells on the right column. Individual panels refer to mRNA levels for the mRNAs actin, LANA, and vCYC. LANA refers to the latency-associated nuclear antigen (orf73) and vCYC to the viral cyclin homolog orf72. Shown for each panel are the concentrations in picogram per milliliter for the indicated cytokines in the vertical axis and time after addition of rapamycin (▪) or vehicle (⊡) on the horizontal axis for the cell lines BC-1 and BCBL-1: (G) hu-IL-6, (H) hu-IL-10, (I) IFN-γ, (J) IP-10, (K) RANTES, (L) MIP-1α, (M) IL12p40. These data represent 1 of 2 biologic replicates. Panel N shows a representative (IL-6) standard curve.